| Expression pattern: |
DN |
| Associated gene: |
p53 protein, ADAR1, HIF-1alpha |
| Associated microRNA: |
- |
| Biological function: |
CircFOXO3 inhibits p53-mediated autophagy and reduces migration and invasion of endometrial stromal cells; hypoxia-mediated downregulation of circFOXO3 promotes autophagy, migration and invasion in endometriosis. |
| Molecular mechanism: |
Hypoxia-induced HIF-1alpha directly binds the ADAR1 promoter and increases ADAR1 expression; ADAR1 binds circFOXO3 pre-mRNA to inhibit circFOXO3 cyclization, and reduced circFOXO3 decreases p53 degradation, thereby promoting autophagy, migration and invasion. |
| Biological pathway or process: |
autophagy (inhibits); migration (inhibits); invasion (inhibits); p53 signaling (inhibits); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Transfection; Western Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; ChIP / ChIP-seq; Transwell Assay; Bioinformatics Analysis |
| Clinical significance: |
circ-FOXO3 might be a possible biomarker for noninvasive diagnosis and a therapeutic target to suppress endometriosis development. |
| Description: |
CircFOXO3 is down-regulated in ectopic endometrium from endometriosis patients. In this study, circFOXO3 overexpression inhibited p53-mediated autophagy and reduced migration and invasion of T HESCs, while hypoxia-induced HIF-1alpha/ADAR1 signaling suppressed circFOXO3 formation and promoted endometriosis-related autophagy. |
| Confidence score: |
0.8187 |