circRNA basic information
circBase ID: -
Name: hsa_circ_TUSC3
Synonym: circ104557
Host Gene: TUSC3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004992
MONDO name: cancer
Disease details: human cancer
Disease DO ID:
 0050686, 0050687, 162
Disease MeSH ID:
 -
Disease NCIt ID:
 C9305
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

normal colon mucosa; tumor tissues
Cell lines:

HCT-116; DKO; Lenti-X 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: -
Associated microRNA: hsa-miR-330-3p
Biological function: inhibits tumor growth; acts as miRNA decoy
Molecular mechanism: Promoter CpG island hypermethylation silences TUSC3 circ104557; circRNA functions as a miRNA decoy reducing hsa-miR-330-3p levels
Biological pathway or process:

ceRNA regulation (promotes); proliferation (inhibits)
Detected method:
Q
M
Validation methods:

Microarray; RNase R Treatment; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Transfection; In Vivo Animal Model
Clinical significance:

-
Description:

TUSC3 circ104557 is epigenetically silenced (downregulated) in methylated HCT-116 cancer cells and reactivated in DNMT1/3B double-knockout cells. Its ectopic expression reduces hsa-miR-330-3p levels (miRNA decoy behavior) and inhibits xenograft tumor growth.
Confidence score:

0.6212
Other information
Title:

Circular RNA CpG island hypermethylation-associated silencing in human cancer.

Journal: Oncotarget
Published: 2018
PubMed ID: 30018746
Study type:

combined biological and clinical study
Data availability: GSE109676
Code availability: -