circRNA basic information
circBase ID: hsa_circ_0000826
Name: hsa_circ_ANKRD12
Synonym: circ_0000826
Host Gene: ANKRD12
Genomic location(hg19): chr18:9182379-9221997:+
Genomic location(hg38): chr18:9182381-9221999:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

serum; CRC tissues; paired adjacent tissues; liver tissue; lung tissue; tumor tissue; colorectal tissues
Cell lines:

HCT116; SW620; 293T
In vivo animal model:

cell line-derived xenograft; genetically engineered animal model; other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: AGO1, AGO2, AGO3, EIF4A3, IGF2BP2, IGF2BP3, LIN28A
Associated microRNA: -
Biological function: Promotes malignant phenotypes (migration, invasion), tumorigenesis, and liver metastasis of colorectal cancer under hypoxia; potential biomarker for CRC liver metastasis.
Molecular mechanism: Hypoxia induces hsa_circ_0000826; genetic knockdown/knockout blocks hypoxia-enhanced migration/invasion and liver metastasis; potential interaction with RNA-binding proteins predicted by computational tool.
Biological pathway or process:

migration (promotes); invasion (promotes); metastasis (promotes); proliferation (promotes); ceRNA regulation (other); EMT (other)
Detected method:
Q
H
Validation methods:

RT-qPCR; ISH (In Situ Hybridization); Clinical Sample Validation; ROC Analysis; Transfection; Transwell Assay; In Vivo Animal Model; H&E Staining
Clinical significance:

Serum hsa_circ_0000826 is higher in CRC patients than healthy donors and higher in CRC patients with liver metastasis than those without; ROC analysis suggests it can discriminate CRC and predict CRC liver metastasis.
Description:

This study shows that hypoxia induces upregulation of hsa_circ_0000826 in CRC cells and patient serum. hsa_circ_0000826 promotes CRC malignant behaviors (migration/invasion), tumorigenesis, and liver metastasis, and its elevated serum levels have diagnostic value for CRC and CRC liver metastasis.
Confidence score:

0.6474
Other information
Title:

Hypoxia-induced hsa_circ_0000826 is linked to liver metastasis of colorectal cancer.

Journal: Journal of clinical laboratory analysis
Published: 2020
PubMed ID: 32633429
Study type:

combined biological and clinical study
Data availability: -
Code availability: -