circRNA basic information
circBase ID: hsa_circ_0102231
Name: hsa_circ_KIAA0586
Synonym: circ_0102231
Host Gene: KIAA0586
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

lung tissues; NSCLC tissues; tumor tissues; adjacent normal tissues

Cell lines:

A549; H1299; H1581; BEAS-2B; HUVEC

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: NOVA2, Ago2
Associated microRNA: miR-635
Biological function: Promotes NSCLC malignant progression by enhancing proliferation and angiogenesis and inhibiting apoptosis; promotes tumor growth in vivo.
Molecular mechanism: Acts as a miRNA sponge (ceRNA) for miR-635 to increase NOVA2 expression and activate the PI3K/AKT signaling pathway.
Biological pathway or process:

PI3K/AKT (promotes); proliferation (promotes); angiogenesis (promotes); apoptosis (inhibits); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

divergent primers PCR; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Tube Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry)

Clinical significance:

circ_0102231 expression was significantly associated with TNM stage, lymph node metastasis and distant metastasis of NSCLC.

Description:

circ_0102231 is up-regulated in NSCLC tissues/cells and is associated with advanced clinicopathological features. Functionally, circ_0102231 promotes NSCLC proliferation and angiogenesis and suppresses apoptosis, and its knockdown inhibits xenograft tumor growth. Mechanistically, circ_0102231 acts as a ceRNA sponge for miR-635 to derepress NOVA2 and activate PI3K/AKT signaling.

Confidence score:

0.8327

Other information
Title:

Circ_0102231 inactivates the PI3K/AKT signaling pathway by regulating the miR-635/NOVA2 pathway to promote the progression of non-small cell lung cancer.

Journal: Thoracic cancer
Published: 2023
PubMed ID: 37864285
Study type:

combined biological and clinical study

Data availability: The data used to support the findings of this study are available from the corresponding author upon request.
Code availability: -