| Expression pattern: |
UP |
| Associated gene: |
TGFBR2 |
| Associated microRNA: |
miR-372-3p |
| Biological function: |
circRNA MFN2 promotes WT cell advancement, proliferation, migration and invasion, and suppresses apoptosis; knockdown of circRNA MFN2 constrains malignant behaviors of WT cells. |
| Molecular mechanism: |
circRNA MFN2 functions through a ceRNA-like circRNA MFN2/miR-372-3p/TGFBR2 axis, targeting miR-372-3p and modulating TGFBR2 via miR-372-3p. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot |
| Clinical significance: |
circRNA MFN2 was expected to offer support as a marker for WT diagnosis and treatment. |
| Description: |
In this study, circRNA MFN2 was up-regulated in Wilms tumor tissues and WiT49 cells. Functional assays showed that knockdown of circRNA MFN2 suppressed WT cell proliferation, migration and invasion and promoted apoptosis. Mechanistically, circRNA MFN2 modulated the miR-372-3p/TGFBR2 axis, supporting its potential role as a diagnostic or therapeutic marker for WT. |
| Confidence score: |
0.7591 |