| Expression pattern: |
UP |
| Associated gene: |
ATG16L1 |
| Associated microRNA: |
hsa-miR-502-5p |
| Biological function: |
hsa_circ_0002371 promotes intracellular BCG/Mtb growth and survival in macrophages and inhibits autophagy in BCG-infected THP-1 cells. |
| Molecular mechanism: |
hsa_circ_0002371 interacts with and facilitates hsa-miR-502-5p expression, enhancing hsa-miR-502-5p-mediated suppression of ATG16L1, thereby inhibiting macrophage autophagy and promoting intracellular mycobacterial survival. |
| Biological pathway or process: |
autophagy (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; IF (Immunofluorescence); Western Blot; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0002371 could serve as a potential diagnostic biomarker for active tuberculosis, with an AUC of 0.8063. |
| Description: |
hsa_circ_0002371 is up-regulated in PBMCs from active tuberculosis patients and in H37Rv- or BCG-infected THP-1 macrophages. It promotes intracellular mycobacterial survival by inhibiting autophagy through the hsa_circ_0002371/hsa-miR-502-5p/ATG16L1 regulatory axis, and it has potential diagnostic biomarker value for ATB. |
| Confidence score: |
0.8411 |