| Expression pattern: |
DN |
| Associated gene: |
KEAP1, NRF2, HDAC4, AGO2 |
| Associated microRNA: |
miR-141-3p |
| Biological function: |
Suppresses LUAD progression by inhibiting tumor growth, proliferation and migration. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge: circKEAP1 binds miR-141-3p to relieve repression on KEAP1, modulating the KEAP1/NRF2/HDAC4 signaling pathway. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); KEAP1/NRF2/HDAC4 signal pathway (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
B
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Northern Blot; Nuclear-Cytoplasmic Fractionation; Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; MTT; EdU Staining; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Low circKEAP1 was associated with unfavorable prognosis and correlated with lymphatic metastasis and tumor stage. |
| Description: |
circKEAP1 (hsa_circRNA_102442) is down-regulated in LUAD and functions as a tumor suppressor. It sponges miR-141-3p to de-repress KEAP1, thereby modulating the KEAP1/NRF2/HDAC4 signaling cascade and inhibiting LUAD cell proliferation/migration and xenograft growth. Low circKEAP1 expression is associated with unfavorable prognosis in LUAD patients. |
| Confidence score: |
0.8942 |