| Expression pattern: |
UP |
| Associated gene: |
MTDH, AGO2, Collagen I, Collagen III |
| Associated microRNA: |
miR-224-5p |
| Biological function: |
circ-SFMBT2 exacerbates bladder dysfunction in KIC; silencing circ-SFMBT2 alleviates bladder dysfunction, increases bladder smooth muscle contractility, up-regulates miR-224-5p, and suppresses MTDH, Collagen I, and Collagen III expression. |
| Molecular mechanism: |
circ-SFMBT2 functions as a ceRNA by competitively binding miR-224-5p, reducing miR-224-5p-mediated inhibition of MTDH and thereby increasing MTDH expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); fibrosis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; Bioinformatics Analysis; RNase R Treatment; RT-qPCR; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); FISH / smFISH; Transfection; Western Blot; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
circ-SFMBT2 was significantly highly expressed in peripheral blood of patients with KIC and the circ-SFMBT2-miR-224-5p-MTDH regulatory network may provide a theoretical basis for diagnosis and treatment of cystitis. |
| Description: |
circ-SFMBT2 is up-regulated in KIC-associated samples and KIC mouse bladder tissues. It acts as a ceRNA for miR-224-5p, relieving miR-224-5p-mediated repression of MTDH and promoting MTDH expression, which contributes to bladder dysfunction and fibrosis-related changes in KIC. Silencing circ-SFMBT2 restores miR-224-5p activity, reduces MTDH/Collagen I/Collagen III expression, and alleviates bladder functional impairment in KIC mice. |
| Confidence score: |
0.8191 |