circRNA basic information
circBase ID: -
Name: hsa_circ_SCMH1
Synonym: circSCMH1 / circular RNA SCMH1
Host Gene: SCMH1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 1060198
MONDO name: ischemic stroke
Disease details: ischemic stroke / IS
Disease DO ID:
 -
Disease MeSH ID:
 -
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

somatosensory cortex; peri-infarct cortex
Cell lines:

bEnd.3; 293 T
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
DN
Associated gene: FTO, UBC13
Associated microRNA: -
Biological function: promotes vascular repair; promotes vessel regeneration and perfusion; repairs BBB integrity following stroke
Molecular mechanism: circSCMH1 binds FTO and promotes its nuclear translocation via UBC13-mediated Ub-K63 ubiquitination, leading to m6A demethylation of Plpp3 mRNA and increased Plpp3/LPP3 expression (enhanced mRNA stability).
Biological pathway or process:

m6A modification (inhibits); mRNA stability (promotes); angiogenesis (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; Transfection; In Vivo Animal Model; IF (Immunofluorescence); Flow Cytometry（Non-apoptosis/cycle）; MeRIP / MeRIP-seq; Actinomycin D / DRB Stability Assay; Bioinformatics Analysis
Clinical significance:

circSCMH1 is decreased in somatosensory cortex of AIS patients versus nonstroke controls.
Description:

circSCMH1 is down-regulated after ischemic stroke and, when supplemented (EV-delivered/overexpressed), promotes vascular repair and BBB restoration. Mechanistically, it binds FTO and enhances UBC13-dependent Ub-K63 ubiquitination and nuclear translocation of FTO, reducing m6A on Plpp3 mRNA, increasing Plpp3/LPP3 expression via improved mRNA stability, and thereby improving stroke recovery.
Confidence score:

0.7198
Other information
Title:

FTO-dependent m6A modification of Plpp3 in circSCMH1-regulated vascular repair and functional recovery following stroke.

Journal: Nature communications
Published: 2023
PubMed ID: 36717587
Study type:

combined biological and clinical study
Data availability: GSE193633
Code availability: -