| Expression pattern: |
UP |
| Associated gene: |
NLRP3, TLR4, MyD88, NF-kappaB, ASC, Caspase-1, GSDMD-N, IL-18 |
| Associated microRNA: |
mmu-miR-193a-5p, miR-193a-5p |
| Biological function: |
circHipk3 promotes pancreatic acinar cell pyroptosis and inflammatory responses in acute pancreatitis; circHipk3 knockdown and QJHGD treatment improve MPC-83 cell viability and reduce pancreatic acinar cell damage. |
| Molecular mechanism: |
circHipk3 functions as a ceRNA sponge for miR-193a-5p, thereby relieving miR-193a-5p-mediated repression of NLRP3 and activating the NLRP3 inflammasome and inflammatory signaling. |
| Biological pathway or process: |
pyroptosis (promotes); inflammation (promotes); NF-kappaB (promotes); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; ELISA; Western Blot; IHC (Immunohistochemistry); IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
In this study, mouse circHipk3 was up-regulated in caerulein-induced acute pancreatitis models. circHipk3 acted as a ceRNA sponge for miR-193a-5p to promote NLRP3 inflammasome activation, pyroptosis, and inflammatory responses; QJHGD and circHipk3 silencing alleviated pancreatic acinar cell injury through this pathway. |
| Confidence score: |
0.705 |