| Expression pattern: |
DN |
| Associated gene: |
TCF7L2, NRAS, CTNNB1, KRAS |
| Associated microRNA: |
- |
| Biological function: |
Serum hsa_circ_0004099 is lowly expressed in the failed sperm retrieval group; its over-expression in serum was reported as beneficial for successful sperm retrieval and it showed predictive value for SRR. |
| Molecular mechanism: |
Predicted ceRNA mechanism; bioinformatics analysis constructed a circRNA-microRNA-mRNA network and predicted enrichment of target genes in spermatogenesis-related pathways. hsa_circ_0004099 was additionally bioinformatically enriched in pathways involving TCF7L2, NRAS, CTNNB1, and KRAS. |
| Biological pathway or process: |
ceRNA regulation (other); Wnt/beta-catenin (other); Hippo/YAP (other); apoptosis (other); p53 signaling (other); MAPK (other); PI3K/AKT/mTOR (other); proliferation (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Serum hsa_circ_0004099 showed significant potential to predict sperm retrieval rate in NOA patients; AUC 0.677 as a single-circRNA panel. |
| Description: |
hsa_circ_0004099 was significantly lower in serum from NOA patients with failed sperm retrieval. Higher serum expression was associated with successful sperm retrieval, and the circRNA contributed to the multi-circRNA SRR prediction panel; bioinformatics linked it to pathways involving TCF7L2, NRAS, CTNNB1, and KRAS. |
| Confidence score: |
0.459 |