| Expression pattern: |
DN |
| Associated gene: |
cyclin D1, CDK6, MMP-2, MMP-9, Ki67, VEGF, E-cadherin |
| Associated microRNA: |
miR-200a, miR-224 |
| Biological function: |
Promotes gastric cancer cell proliferation and migration; knockdown arrests cell cycle at G0/G1 and inhibits tumour growth in xenografts. |
| Molecular mechanism: |
Knockdown decreases cyclin D1/CDK6 and MMP-2/MMP-9 protein levels; proposed ceRNA interaction with miR-200a and miR-224. |
| Biological pathway or process: |
cell cycle (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Clinical Sample Validation; ROC Analysis; Transfection; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining |
| Clinical significance: |
Potential diagnostic biomarker for gastric cancer (AUC=0.82); associated with invasion and TNM stage. |
| Description: |
hsa_circ_0000096 is significantly down-regulated in gastric cancer tissues and cell lines and has diagnostic potential (ROC AUC 0.82). Functional knockdown experiments indicate it supports cell-cycle progression, proliferation, and migration, accompanied by reduced cyclin D1/CDK6 and MMP-2/MMP-9; a ceRNA relationship with miR-200a/miR-224 is suggested. |
| Confidence score: |
0.722 |