| Expression pattern: |
DN |
| Associated gene: |
FOXO3a, NLRP3 |
| Associated microRNA: |
miR-138-5p |
| Biological function: |
Circ DENND4C alleviates ischemia-reperfusion acute kidney injury, enhances HK-2 cell proliferation, inhibits NLRP3 activation, reduces pyroptosis and inflammation, and protects renal function. |
| Molecular mechanism: |
Exosomal circ DENND4C acts as a sponge for miR 138-5p and regulates FOXO3a, thereby inhibiting NLRP3-related pyroptosis and alleviating AKI. |
| Biological pathway or process: |
pyroptosis (inhibits); proliferation (promotes); inflammation (inhibits); ceRNA regulation (other); other pathway/process (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNA-seq; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); ELISA |
| Clinical significance: |
Circ DENND4C may be a potential therapeutic target for AKI. |
| Description: |
Circ DENND4C is decreased in ischemia-reperfusion AKI and is enriched in human USC-derived exosomes. Exosomal circ DENND4C protects against renal I/R injury by sponging miR 138-5p and regulating FOXO3a, which promotes cell proliferation and suppresses NLRP3-associated pyroptosis and inflammation. |
| Confidence score: |
0.6829 |