| Expression pattern: |
UP |
| Associated gene: |
FRS2, CyclinD1, Vimentin, Ago2 |
| Associated microRNA: |
miR-424-5p |
| Biological function: |
Promotes ox-LDL-induced VSMC viability, proliferation, invasion and migration; circ_0004872 knockdown restrains these ox-LDL-stimulated VSMC dysfunction phenotypes. |
| Molecular mechanism: |
circ_0004872 acts as a ceRNA sponge for miR-424-5p, thereby elevating FRS2 expression and promoting ox-LDL-induced VSMC dysfunction. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Western Blot; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ_0004872 was elevated in AS patient serum and had diagnostic value by ROC analysis; it may be a potential molecular target for AS treatment. |
| Description: |
circ_0004872 is up-regulated in serum from AS patients and in ox-LDL-treated VSMCs. It promotes ox-LDL-induced VSMC proliferation, invasion and migration by sponging miR-424-5p and increasing FRS2 expression, suggesting diagnostic and therapeutic relevance in AS. |
| Confidence score: |
0.817 |