circRNA basic information
circBase ID: hsa_circ_0037104
Name: hsa_circ_PCSK6
Synonym: circ_0037104
Host Gene: PCSK6
Genomic location(hg19): chr15:101968096-101972305:-
Genomic location(hg38): chr15:101427891-101432100:-
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0019087
MONDO name: cholangiocarcinoma
Disease details: cholangiocarcinoma
Disease DO ID:
 4947
Disease MeSH ID:
 D018281
Disease NCIt ID:
 C4436
Disease ICD11 ID:
 2110597275
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

CCA tissues; paracancerous tissues; human umbilical cords; subcutaneous tumors
Cell lines:

TFK-1; HuCCT1; HiBEpiC; Hu-MSCs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: AFAP1, APAF1, PCSK6
Associated microRNA: miR-620
Biological function: circ_0037104 inhibits CCA cell proliferation, migration, invasion, metastasis, malignant progression, EMT, and stemness.
Molecular mechanism: Hu-MSC-derived exosomal circ_0037104 functions as a sponge for miR-620 and regulates the miR-620/AFAP1 axis, thereby enhancing APAF1/AFAP1 expression and suppressing CCA progression.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); stemness (inhibits); ceRNA regulation (other)
Detected method:
Q
S
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; IHC (Immunohistochemistry); Western Blot; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

potentially acting as a biomarker for CCA diagnosis
Description:

circ_0037104 is downregulated in cholangiocarcinoma and is enriched in Hu-MSC-derived exosomes. It suppresses CCA proliferation, migration, invasion, metastasis, EMT, and stemness by sponging miR-620 and regulating the AFAP1/APAF1 axis. The study suggests that Hu-MSC exosomal circ_0037104 may have diagnostic biomarker and therapeutic relevance in CCA.
Confidence score:

0.8634
Other information
Title:

Exosomal circ_0037104 derived from Hu-MSCs inhibits cholangiocarcinoma progression by sponging miR-620 and targeting AFAP1.

Journal: Journal of biochemical and molecular toxicology
Published: 2024
PubMed ID: 38348717
Study type:

combined biological and clinical study
Data availability: All raw data supporting the conclusions of this article are available from authors upon request.
Code availability: -