circRNA basic information
circBase ID: hsa_circ_0029343
Name: hsa_circ_SCARB1
Synonym: circ _0029343
Host Gene: SCARB1
Genomic location(hg19): chr12:125292306-125302253:-
Genomic location(hg38): chr12:124807760-124817707:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue; corresponding adjacent normal tissue; liver cancer tissue; tumor tissues
Cell lines:

HCCLM3; Huh-7; Huh-1; LX-2; THLE-2; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SRSF3, p73, PDGF-PDGFRB, PDGFRB, AKT, ERK
Associated microRNA: miR-486-5p
Biological function: Promotes proliferation, migration, invasion, tumor growth and metastasis, and inhibits apoptosis of HCC cells.
Molecular mechanism: circ_0029343 sponges miR-486-5p to up-regulate SRSF3, induces p73 splicing, and activates the PDGF-PDGFRB signaling pathway.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); ceRNA regulation (promotes); ERK (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; IF (Immunofluorescence); In Vivo Animal Model
Clinical significance:

-
Description:

SCARB1-encoded circ_0029343 is up-regulated in HCC tissues and cell lines. It promotes HCC cell proliferation, migration, invasion, growth and metastasis while suppressing apoptosis, mainly by sponging miR-486-5p, increasing SRSF3, inducing p73 splicing, and activating PDGF-PDGFRB-related signaling.
Confidence score:

0.6545
Other information
Title:

SCARB1-encoded circ _0029343 induces p73 splicing to promote growth and metastasis of hepatocellular carcinoma via miR-486-5p/SRSF3 axis.

Journal: Journal of biochemical and molecular toxicology
Published: 2024
PubMed ID: 38345168
Study type:

combined biological and clinical study
Data availability: GSE108724; GSE168852
Code availability: -