circRNA basic information
circBase ID: hsa_circ_0004277
Name: hsa_circ_WDR37
Synonym: circ-0004277
Host Gene: WDR37
Genomic location(hg19): chr10:1125950-1126416:+
Genomic location(hg38): chr10:1080010-1080476:+
Subcellular localization: exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue; adjacent normal tissues; plasma exosomes
Cell lines:

HL-7702; HepG2; Bel-7402; MHCC97; Huh-7; SMMC-7721; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HuR, ZO-1, ZO-1 mRNA
Associated microRNA: -
Biological function: Promotes proliferation, migration, invasion/metastasis and EMT progression of HCC cells; exosomal circ-0004277 stimulates EMT and migration of surrounding normal hepatic cells.
Molecular mechanism: circ-0004277 competitively binds the RBP HuR, blocks HuR binding to ZO-1 mRNA, inhibits ZO-1, and thereby stimulates EMT progression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); other pathway/process (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Sanger Sequencing; Clinical Sample Validation; ROC Analysis; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; RIP (RNA Immunoprecipitation); Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis
Clinical significance:

Exosomal circ-0004277 may be a diagnostic biomarker for distinguishing HCC patients from negative controls (ROC AUC = 0.816).
Description:

hsa_circ_0004277 (circ-0004277), derived from WDR37, is up-regulated in HCC tissues, HCC cell lines, and plasma exosomes, and promotes HCC proliferation, migration, EMT, and metastasis. Mechanistically, it binds the RBP HuR, interferes with HuR-ZO-1 mRNA interaction, suppresses ZO-1, and thereby enhances EMT. Exosomal circ-0004277 can be transferred to surrounding normal hepatic cells to stimulate EMT/migration, and it shows diagnostic potential by ROC analysis.
Confidence score:

0.8202
Other information
Title:

Circular RNA hsa_circ_0004277 Stimulates Malignant Phenotype of Hepatocellular Carcinoma and Epithelial-Mesenchymal Transition of Peripheral Cells.

Journal: Frontiers in cell and developmental biology
Published: 2020
PubMed ID: 33511111
Study type:

combined biological and clinical study
Data availability: https://www.frontiersin.org/articles/10.3389/fcell.2020.585565/full#supplementary-material
Code availability: -