circRNA basic information
circBase ID: hsa_circ_0000735
Name: hsa_circ_P2RX1
Synonym: circ_0000735
Host Gene: P2RX1
Genomic location(hg19): chr17:3802927-3808661:-
Genomic location(hg38): chr17:3899633-3905367:-
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; adjacent normal tissues; serum; serum exosomes; tumor tissues
Cell lines:

A549; H1299; BEAS-2B
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ADAM19, P2RX1
Associated microRNA: miR-345-5p
Biological function: circ_0000735 promotes NSCLC cell proliferation, migration, invasion, metastasis, EMT and tumorigenesis; exosomal circ_0000735 mediates intercellular communication and may serve as a serum diagnostic biomarker.
Molecular mechanism: circ_0000735 acts as a miRNA sponge for miR-345-5p to upregulate ADAM19; exosomes mediate intercellular transmission of circ_0000735.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; EdU Staining; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis
Clinical significance:

Serum exosomal circ_0000735 may be a potential biomarker for NSCLC diagnosis and for determination of metastasis.
Description:

circ_0000735 is upregulated in NSCLC tissues, cells, and serum exosomes and acts as an oncogenic circRNA. It promotes NSCLC proliferation, migration, invasion, EMT, metastasis, and tumorigenesis by sponging miR-345-5p to regulate ADAM19, while exosomal circ_0000735 supports intercellular communication and may serve as a diagnostic biomarker.
Confidence score:

0.7855
Other information
Title:

Exosomal circ_0000735 contributes to non-small lung cancer malignant progression.

Journal: Journal of biochemical and molecular toxicology
Published: 2024
PubMed ID: 38528705
Study type:

combined biological and clinical study
Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Code availability: -