| Expression pattern: |
UP |
| Associated gene: |
APP |
| Associated microRNA: |
hsa-miR-3612, hsa-miR-3064-5p, hsa-miR-542-3p, hsa-miR-4731-5p, hsa-miR-2467-3p, hsa-miR-5691, hsa-miR-338-3p, hsa-miR-185-5p |
| Biological function: |
circRNA_0023685 promotes gastric cancer cell proliferation, migration and tumor growth, and its downregulation increases spontaneous apoptosis in MKN45 cells. |
| Molecular mechanism: |
circRNA_0023685 may act as a miRNA sponge and promote gastric cancer through a circRNA-miRNA-mRNA network involving APP and cell division-related genes. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes); immune regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; ROC Analysis; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circRNA_0023685 may serve as a new clinical diagnostic biomarker and therapeutic target for gastric cancer. |
| Description: |
circRNA_0023685 is upregulated in gastric cancer plasma, tissues and cell lines. Functional knockdown experiments indicate that it promotes GC proliferation, migration and xenograft tumor growth while suppressing apoptosis. Mechanistically, the study proposes a circRNA-miRNA-mRNA ceRNA network in which circRNA_0023685 regulates APP, supporting its potential as a diagnostic biomarker and therapeutic target. |
| Confidence score: |
0.6772 |