| Expression pattern: |
DN |
| Associated gene: |
ITGA5, RSU1 |
| Associated microRNA: |
miR-1224-5p |
| Biological function: |
Alleviates LPS-induced HPMEC injury by reducing inflammatory response, restoring migration and tube formation, decreasing cell permeability, and preserving tight junction markers. |
| Molecular mechanism: |
circRSU1 functions as a ceRNA sponge for miR-1224-5p and regulates ITGA5 expression through the miR-1224-5p/ITGA5 axis. |
| Biological pathway or process: |
ceRNA regulation (other); inflammation (inhibits); migration (promotes); angiogenesis (promotes); other pathway/process (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; Transwell Assay; Wound Healing Assay; Tube Formation Assay; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential clinical application in sepsis-ALI prevention. |
| Description: |
This study found that circRSU1 is down-regulated in LPS-induced HPMECs, an in vitro model of sepsis-ALI. Restoring circRSU1 alleviated endothelial injury by suppressing inflammatory cytokine changes, promoting migration and tube formation, reducing permeability, and maintaining tight junction proteins. Mechanistically, circRSU1 acts through the miR-1224-5p/ITGA5 axis. |
| Confidence score: |
0.7216 |