| Expression pattern: |
UP |
| Associated gene: |
CYP1A1 |
| Associated microRNA: |
- |
| Biological function: |
Promotes abnormal proliferation, colony formation, cell cycle progression, BaP-induced malignant transformation, BPDE-DNA adduct formation and DNA damage, and inhibits apoptosis in human bronchial epithelial cells. |
| Molecular mechanism: |
circ0087385 upregulates CYP1A1 expression, increases BaP metabolic activation and BPDE-DNA adduct formation, and thereby promotes DNA damage and malignant transformation. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; TUNEL; Western Blot; IF (Immunofluorescence); ELISA; Bioinformatics Analysis |
| Clinical significance: |
circ0087385 was significantly overexpressed in lung cancer tissues and serum exosomes from lung cancer cases, suggesting potential relevance to lung cancer detection or intervention targets. |
| Description: |
circ0087385 is a human AGTPBP1-derived circRNA that is upregulated after BaP exposure and in lung cancer cells, tissues, and serum exosomes. It promotes BaP-induced DNA damage and malignant transformation by upregulating CYP1A1, increasing BPDE-DNA adduct formation, enhancing proliferation and cell cycle progression, and suppressing apoptosis. |
| Confidence score: |
0.7471 |