| Expression pattern: |
DN |
| Associated gene: |
HNRNPC, ITCH, Ki-67 |
| Associated microRNA: |
miR-224-3p |
| Biological function: |
circITCH acts as a tumor suppressor in NPC; circITCH overexpression inhibits NPC cell proliferation, migration and invasion, and promotes apoptosis. |
| Molecular mechanism: |
circITCH suppresses NPC progression by sponging miR-224-3p, while the m6A reader/RBP HNRNPC directly interacts with circITCH and reduces circITCH expression. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); ceRNA regulation (other); m6A modification (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Transfection; CCK8; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot |
| Clinical significance: |
circITCH can be used as a biomarker to predict NPC development and increased circITCH expression could be a potential treatment for NPC. |
| Description: |
circITCH is down-regulated in NPC cell lines and functions as a tumor-suppressive circRNA. Its overexpression suppresses proliferation, migration and invasion while promoting apoptosis, mainly by sponging miR-224-3p; HNRNPC directly interacts with circITCH and is proposed to reduce circITCH expression in an m6A-related regulatory context. |
| Confidence score: |
0.627 |