| Expression pattern: |
DN |
| Associated gene: |
RORA, Ago2 |
| Associated microRNA: |
miR-1290 |
| Biological function: |
Inhibits glioma malignant phenotypes (proliferation, migration, invasion) and promotes apoptosis and cell cycle arrest; mediates sevoflurane anti-glioma effects and suppresses tumor growth in vivo. |
| Molecular mechanism: |
circRELN acts as a miRNA sponge for miR-1290 to de-repress RORA (ceRNA axis circRELN/miR-1290/RORA). |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); cell cycle (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNA-seq; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RNase R Treatment; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
circRELN is down-regulated in glioma tissues and cell lines, and sevoflurane increases its expression. circRELN suppresses glioma malignant behaviors and tumor growth by sponging miR-1290 and thereby de-repressing RORA (circRELN/miR-1290/RORA axis). |
| Confidence score: |
0.8279 |