| Expression pattern: |
DN |
| Associated gene: |
TOB2, PLIN3 |
| Associated microRNA: |
miR-661 |
| Biological function: |
Acts as a tumor suppressor in ccRCC; inhibits cell proliferation, migration, invasion, metastasis, EMT-associated marker changes, tumor formation, and lipid accumulation. |
| Molecular mechanism: |
hsa_circ_0086414 acts as a miR-661 sponge to upregulate TOB2, and the hsa_circ_0086414/TOB2 axis inhibits PLIN3 to reduce lipid deposition and suppress ccRCC progression. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); cell cycle (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Oil Red O Staining; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0086414 is downregulated in clinical ccRCC samples and may be a potential therapeutic target via the hsa_circ_0086414/miR-661/TOB2/PLIN3 axis. |
| Description: |
hsa_circ_0086414 is downregulated in ccRCC tissues and cells and functions as a tumor suppressor. It sponges miR-661 to increase TOB2 expression, leading to PLIN3 inhibition and reduced ccRCC proliferation, metastasis, EMT-related changes, and lipid accumulation. |
| Confidence score: |
0.8455 |