circRNA basic information
circBase ID: hsa_circ_0003731
Name: hsa_circ_PTGR1
Synonym: circPTGR1
Host Gene: PTGR1
Genomic location(hg19): chr9:114341075-114348445:-
Genomic location(hg38): chr9:111578795-111586165:-
Subcellular localization: exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

serum exosomes

Cell lines:

HepG2; 97L; LM3; 97H; L-O2; SMCC-7721; HEP3B; HUH7

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: MET
Associated microRNA: miR449a
Biological function: promotes migration and invasion; promotes metastasis/progression of HCC
Molecular mechanism: ceRNA mechanism involving miR449a and MET
Biological pathway or process:

migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes)

Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Luciferase Reporter Assay; Transfection; Transwell Assay; In Vivo Animal Model; H&E Staining; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis

Clinical significance:

Associated with clinical stage and prognosis in HCC; high circPTGR1 indicates worse disease-free survival

Description:

hsa_circ_0003731 (circPTGR1 isoform) is upregulated in metastatic HCC cell/exosome contexts and in patient serum exosomes, and is linked to stage and poor prognosis. Functionally, circPTGR1 promotes HCC migration/invasion and metastasis through a miR449a-dependent increase of MET.

Confidence score:

0.814

Other information
Title:

Three isoforms of exosomal circPTGR1 promote hepatocellular carcinoma metastasis via the miR449a-MET pathway.

Journal: EBioMedicine
Published: 2019
PubMed ID: 30630697
Study type:

combined biological and clinical study

Data availability: NCBI: SRP165940
Code availability: -