circRNA basic information
circBase ID: hsa_circ_0006948
Name: hsa_circ_FNDC3B
Synonym: Circ_FNDC3B
Host Gene: FNDC3B
Genomic location(hg19): chr3:171830241-171944737:+
Genomic location(hg38): chr3:172112451-172226947:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005580
MONDO name: esophageal squamous cell carcinoma
Disease details: esophageal squamous cell carcinoma
Disease DO ID:
 3748
Disease MeSH ID:
 C562729; D000077277
Disease NCIt ID:
 C4024
Disease ICD11 ID:
 1417891145
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

ESCC tissue samples; corresponding para-carcinoma tissues; tumor tissues
Cell lines:

EC9706; KYSE30; TE-8; KYSE450; HET-1A
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MAPK1, FNDC3B, Ki67, BCL2, BAX
Associated microRNA: miR-136-5p
Biological function: Promotes ESCC cell proliferation, migration, invasion, EMT, metastasis and tumor growth, while inhibiting apoptosis.
Molecular mechanism: circ_FNDC3B functions as a miR-136-5p sponge to relieve miR-136-5p-mediated repression of MAPK1, thereby promoting ESCC progression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

circ_FNDC3B upregulation was related to T-stage III, lymph node metastasis, and poor prognosis.
Description:

circ_FNDC3B is up-regulated in ESCC tissues and cells and acts as an oncogenic circRNA. Its knockdown suppresses proliferation, migration, invasion, EMT and xenograft tumor growth while promoting apoptosis. Mechanistically, circ_FNDC3B sponges miR-136-5p to increase MAPK1 expression, forming a circ_FNDC3B/miR-136-5p/MAPK1 axis associated with malignant ESCC progression.
Confidence score:

0.7859
Other information
Title:

Circ_FNDC3B Promotes Cell Proliferation and Metastasis in Esophageal Squamous Cell Carcinoma via Regulating MAPK1 by Binding to miR-136-5p.

Journal: Biochemical genetics
Published: 2024
PubMed ID: 38228844
Study type:

combined biological and clinical study
Data availability: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -