| Expression pattern: |
UP |
| Associated gene: |
FSCN1, AGO2 |
| Associated microRNA: |
miR-326 |
| Biological function: |
promotes proliferation, migration and invasion of NSCLC cells; induces abnormal proliferation in normal bronchial epithelial cells via exosomes; associated with lymphatic metastasis |
| Molecular mechanism: |
ceRNA-like mechanism: circSATB2 binds miR-326 and negatively regulates miR-326, thereby increasing FSCN1 expression; circSATB2 is transferred via exosomes to affect recipient cells |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; EdU Staining; Wound Healing Assay; Transwell Assay; CCK8; Colony Formation Assay; Western Blot; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Exosomal circSATB2 is highly expressed in serum exosomes from NSCLC patients and shows diagnostic potential (ROC/AUC); associated with lymphatic metastasis |
| Description: |
circSATB2 is up-regulated in NSCLC tissues/cells and in serum exosomes from NSCLC patients, and promotes NSCLC proliferation, migration and invasion. Mechanistically, it mainly localizes to the cytoplasm, sponges/negatively regulates miR-326, and thereby upregulates FSCN1; it can also be transferred via exosomes to affect recipient cells. Serum exosomal circSATB2 shows diagnostic potential and correlates with lymphatic metastasis. |
| Confidence score: |
0.8399 |