| Expression pattern: |
DN |
| Associated gene: |
IRAK1, TRAF6, Ago2 |
| Associated microRNA: |
miR-146a |
| Biological function: |
Promotes C. acnes biofilm-induced inflammatory response in human primary keratinocytes; increases TNF-alpha, IL-6 and IL-8 levels; promotes MAPK and NF-kappaB pathway activation. |
| Molecular mechanism: |
Acts as a ceRNA that sponges miR-146a, suppresses miR-146a expression, and thereby promotes IRAK1 and TRAF6 expression to activate inflammatory signaling through MAPK and NF-kappaB pathways. |
| Biological pathway or process: |
inflammation (promotes); MAPK (promotes); NF-kappaB (promotes); ceRNA regulation (other) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; Western Blot; IF (Immunofluorescence) |
| Clinical significance: |
Potential therapeutic target and new marker for clinical treatment of acne. |
| Description: |
This study identifies hsa_circ_0105040 as a down-regulated FLNA-derived circRNA in acne tissues that is predominantly localized in the cytoplasm of human primary keratinocytes. Functionally, hsa_circ_0105040 promotes C. acnes biofilm-induced inflammation by sponging miR-146a, thereby increasing IRAK1/TRAF6 expression and activating MAPK and NF-kappaB-related inflammatory responses. The authors propose this hsa_circ_0105040/miR-146a/IRAK1/TRAF6 axis as a potential therapeutic target or marker for inflammatory acne. |
| Confidence score: |
0.8304 |