| Expression pattern: |
UP |
| Associated gene: |
HOXC8 |
| Associated microRNA: |
miR-502-5p |
| Biological function: |
promotes proliferation and migration of bladder cancer cells; promotes tumor growth in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-502-5p to increase HOXC8 mRNA and protein expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circNT5E expression was positively associated with larger tumor size and lower overall survival rate in bladder cancer patients. |
| Description: |
circNT5E is up-regulated in bladder cancer tissues and promotes bladder cancer cell proliferation and migration. Mechanistically, it localizes mainly in the cytoplasm and sponges miR-502-5p to upregulate HOXC8, enhancing tumor growth in vitro and in a xenograft model; high circNT5E is associated with larger tumor size and worse overall survival. |
| Confidence score: |
0.817 |