| Expression pattern: |
UP |
| Associated gene: |
Gprc5a |
| Associated microRNA: |
- |
| Biological function: |
Promotes bladder cancer stem cell self-renewal and metastasis/invasion; supports tumor propagation in vivo. |
| Molecular mechanism: |
circGprc5a encodes a peptide (circGprc5a-peptide) that binds the membrane protein Gprc5a and activates GPCR signaling in a peptide-dependent, Gprc5a-dependent manner. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); metastasis (promotes); stemness (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; ISH (In Situ Hybridization); FISH / smFISH; Clinical Sample Validation; Survival Analysis; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; Western Blot; RNA Pull-Down; Bioinformatics Analysis; Flow Cytometry（Non-apoptosis/cycle） |
| Clinical significance: |
High circGprc5a expression is associated with advanced/metastatic bladder tumors and worse clinical outcome. |
| Description: |
circGprc5a is upregulated in bladder cancer and bladder CSCs and promotes CSC maintenance, self-renewal, invasion/metastasis and tumor propagation. Mechanistically, it encodes a peptide that binds Gprc5a and functions in a peptide- and Gprc5a-dependent manner, implicating GPCR signaling and providing a potential therapeutic targeting axis. |
| Confidence score: |
0.749 |