| Expression pattern: |
UP |
| Associated gene: |
FUT8 |
| Associated microRNA: |
miR-548c |
| Biological function: |
promotes HCC development and progression by promoting proliferation and invasion, supporting metastasis, inducing cell cycle progression, and being associated with EMT |
| Molecular mechanism: |
acts as an RNA sponge for miR-548c, thereby attenuating miR-548c inhibition of FUT8 and indirectly increasing FUT8 expression |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); metastasis (promotes); cell cycle (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; ISH (In Situ Hybridization); FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Cell Cycle Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; Survival Analysis |
| Clinical significance: |
cFUT8 expression was negatively associated with the prognosis of patients with HCC and correlated with BCLC stage and lymphatic invasion |
| Description: |
cFUT8 is up-regulated in HCC and promotes malignant phenotypes (proliferation, invasion, metastasis, and EMT-related changes). Mechanistically, it predominantly localizes in the cytoplasm and sponges miR-548c, relieving miR-548c-mediated repression of FUT8 and thereby increasing FUT8 expression; high cFUT8 is associated with worse survival. |
| Confidence score: |
0.7859 |