| Expression pattern: |
UP |
| Associated gene: |
MTBP, MAN1A2 |
| Associated microRNA: |
miR-502-5p |
| Biological function: |
promotes GC cell proliferation, migration, tumor growth and metastasis |
| Molecular mechanism: |
circ_0000119 acts as a miRNA sponge for miR-502-5p to upregulate MTBP expression, thereby promoting GC cell proliferation and migration |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); metastasis (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0000119 silencing would be a promising therapeutic target in GC patients |
| Description: |
circ_0000119 is an exonic MAN1A2-derived circRNA that is up-regulated in gastric cancer tissues and cell lines. It promotes gastric cancer cell proliferation, migration, tumor growth and lung metastasis by sponging miR-502-5p and increasing MTBP expression. The study suggests circ_0000119 silencing may be a therapeutic strategy for GC patients. |
| Confidence score: |
0.8138 |