| Expression pattern: |
DN |
| Associated gene: |
- |
| Associated microRNA: |
miR-762, miR-920, hsa-miR-1183, hsa-miR-323b-5p |
| Biological function: |
circLPAR1 is down-regulated in MIBC and functions as a potential prognostic biomarker; knockdown of circLPAR1 enhances bladder cancer cell invasion and wound-healing ability, suggesting that circLPAR1 suppresses invasion and metastasis. |
| Molecular mechanism: |
circLPAR1 binds to miR-762 and inhibits its activity as a miRNA sponge; loss of circLPAR1 enhances invasion, and miR-762 inhibitor attenuates the si-circLPAR1-induced increase in invasion. |
| Biological pathway or process: |
invasion (inhibits); migration (inhibits); metastasis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; Cohort Study; Survival Analysis |
| Clinical significance: |
Low circLPAR1 expression predicted poor disease-specific survival in MIBC patients; circLPAR1 may be a potential novel and stable prognostic biomarker. |
| Description: |
circLPAR1/hsa_circ_0087960 is a human LPAR1-derived circRNA down-regulated in MIBC tissues. Higher circLPAR1 expression is associated with better disease-specific survival, while knockdown enhances bladder cancer cell invasion and migration. Mechanistically, circLPAR1 binds miR-762 and inhibits its activity, suggesting a tumor-suppressive miRNA-sponge role in invasion and metastasis. |
| Confidence score: |
0.8267 |