| Expression pattern: |
UP |
| Associated gene: |
CYP24A1 |
| Associated microRNA: |
hsa-miR-224-3p, hsa-miR-522-3p, hsa-miR-1264 |
| Biological function: |
Potential diagnostic biomarker for colorectal cancer; induced by vitamin D treatment; acts as a miRNA sponge to increase CYP24A1 expression |
| Molecular mechanism: |
ceRNA mechanism; miRNA sponge activity; transcriptional regulation related to vitamin D response |
| Biological pathway or process: |
ceRNA regulation (promotes); mRNA stability (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Could serve as a potential diagnostic marker for CRC, but the diagnostic role could be rejected because these transcripts are not expressed in many samples; hsa_circ_0060927 expression level was correlated with gender. |
| Description: |
This study reports that hsa_circ_0060927 is up-regulated in a subset of CRC tissues and is inducible by vitamin D in CRC cell lines. It may function as a ceRNA/miRNA sponge for hsa-miR-224-3p, hsa-miR-522-3p, and hsa-miR-1264, thereby increasing CYP24A1 expression, and it was explored as a potential diagnostic biomarker with limited clinical utility due to low detectability in many samples. |
| Confidence score: |
0.6238 |