| Expression pattern: |
UP |
| Associated gene: |
SRSF1, GCH1 |
| Associated microRNA: |
- |
| Biological function: |
circSEPT9 reduces DDP chemosensitivity of TNBC cells, inhibits ferroptosis, increases colony formation, and stabilizes GCH1 protein by blocking GCH1 ubiquitination. |
| Molecular mechanism: |
SRSF1 binds to and upregulates circSEPT9; circSEPT9 binds GCH1 and blocks GCH1 ubiquitination, increasing GCH1 protein stability, thereby inhibiting ferroptosis and reducing cisplatin chemosensitivity. |
| Biological pathway or process: |
ferroptosis (inhibits); chemoresistance (promotes); proliferation (promotes); ubiquitination (inhibits); drug resistance (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
In TNBC cells, circSEPT9 is upregulated, especially in DDP-resistant cells. SRSF1 binds and promotes circSEPT9, while circSEPT9 binds GCH1 and blocks its ubiquitination, stabilizing GCH1 protein. This circSEPT9/GCH1 mechanism inhibits ferroptosis and reduces cisplatin chemosensitivity. |
| Confidence score: |
0.7363 |