circRNA basic information
circBase ID: hsa_circ_0007255
Name: hsa_circ_KIF4A
Synonym: circKIF4A
Host Gene: KIF4A
Genomic location(hg19): chrX:69549254-69553539:+
Genomic location(hg38): chrX:70329404-70333689:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005494
MONDO name: triple-negative breast carcinoma
Disease details: triple-negative breast cancer
Disease DO ID:
 0060081
Disease MeSH ID:
 D064726
Disease NCIt ID:
 C71732
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary TNBC tumor tissues; brain metastasis tissues; brain metastatic tissues
Cell lines:

MCF10A; BT549; MDA-MB-361; MDA-MB-453; MDA-MB-; MDA-MB-468; SUM-159; HCC-1806; HCC1806; SUM159PT
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: STAT3, p62, LC3B, Beclin
Associated microRNA: miR-637
Biological function: Promotes TNBC cell proliferation, migration, invasion, and brain metastasis; inhibits autophagy through the circKIF4A/miR-637/STAT3 axis.
Molecular mechanism: circKIF4A acts as a ceRNA sponge for miR-637, relieving miR-637-mediated inhibition of STAT3 and modulating autophagy-related proteins.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); autophagy (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Western Blot; Bioinformatics Analysis
Clinical significance:

circKIF4A can be used as a prognostic biomarker for brain metastasis in TNBC and as a therapeutic target; promising biomarker for diagnosis and therapy in TNBC brain metastases.
Description:

circKIF4A is up-regulated in TNBC brain metastases and TNBC cell lines. It promotes TNBC proliferation, migration, invasion, tumor growth, and brain metastasis by sponging miR-637 to up-regulate STAT3, thereby modulating autophagy-related signaling. The study proposes circKIF4A as a potential biomarker and therapeutic target for TNBC brain metastasis.
Confidence score:

0.7955
Other information
Title:

A novel axis of circKIF4A-miR-637-STAT3 promotes brain metastasis in triple-negative breast cancer.

Journal: Cancer letters
Published: 2024
PubMed ID: 38029538
Study type:

combined biological and clinical study
Data availability: Supplementary data: https://doi.org/10.1016/j.canlet.2023.216508
Code availability: -