circRNA basic information
circBase ID: hsa_circ_0000673
Name: hsa_circ_RSL1D1
Synonym: -
Host Gene: RSL1D1
Genomic location(hg19): chr16:11940357-11940700:-
Genomic location(hg38): chr16:11846500-11846843:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; tumor and adjacent normal tissues; xenograft tumor tissues

Cell lines:

DLD-1; RKO; HT-29; NCM460

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: CPSF6
Associated microRNA: miR-548b-3p
Biological function: Promotes CRC cell proliferation, migration, invasion, stemness and xenograft tumor growth; silencing circ_0000673 reduces these malignant phenotypes.
Molecular mechanism: circ_0000673 functions as a ceRNA by sponging miR-548b-3p to upregulate CPSF6 expression in CRC cells.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); stemness (promotes)

Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; In Vivo Animal Model; Survival Analysis; ROC Analysis; Bioinformatics Analysis

Clinical significance:

High circ_0000673 expression was associated with poorer overall survival, and ROC curve analysis indicated clinical prognostic significance and potential as a diagnostic biomarker.

Description:

circ_0000673 is up-regulated in colorectal cancer tissues and cells and acts as a tumor-promoting circRNA. Mechanistically, it sponges miR-548b-3p to increase CPSF6 expression, thereby promoting CRC cell proliferation, migration, invasion, stemness and xenograft tumor growth. Clinically, high circ_0000673 expression is associated with poorer overall survival and may have prognostic or diagnostic biomarker value.

Confidence score:

0.8201

Other information
Title:

Inhibiting circ_0000673 blocks the progression of colorectal cancer through downregulating CPSF6 via targeting miR-548b-3p.

Journal: Advances in clinical and experimental medicine : official organ Wroclaw Medical University
Published: 2025
PubMed ID: 39225594
Study type:

combined biological and clinical study

Data availability: https://doi.org/10.5281/zenodo.10867861; available from the corresponding author on reasonable request
Code availability: -