| Expression pattern: |
UP |
| Associated gene: |
SSBP1, CDK2, cyclin E1, Rb, E2F1 |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation, cell cycle G1/S transition, migration, invasion, and tumor growth in cervical cancer |
| Molecular mechanism: |
circZFR binds SSBP1 and promotes assembly/activation of the SSBP1/CDK2/cyclin E1 complex, increasing p-Rb (S807/S608) phosphorylation, releasing E2F1 and driving G1/S transition |
| Biological pathway or process: |
cell cycle (promotes); proliferation (promotes); migration (promotes); invasion (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; circRIP; RNA Pull-Down; Co-IP; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); ROC Analysis |
| Clinical significance: |
Increased expression of circZFR was positively associated with lymphatic metastasis, SCC Ag value, and Ki67 value; ROC AUC for distinguishing cervical cancer from normal was 0.88. |
| Description: |
circZFR (hsa_circ_0072088), derived from ZFR, is up-regulated in cervical cancer and promotes proliferation, G1/S transition, migration/invasion, and xenograft tumor growth. Mechanistically it binds the RBP SSBP1 to enhance SSBP1/CDK2/cyclin E1 complex assembly, increasing Rb phosphorylation (S807/S608) and activating E2F1-driven cell-cycle programs. |
| Confidence score: |
0.8712 |