| Expression pattern: |
UP |
| Associated gene: |
MYC, TIP60, FUBP1, FIR, CCNE1, CDK4 |
| Associated microRNA: |
- |
| Biological function: |
Promotes breast cancer cell proliferation, migration, invasion, cell cycle G1/S progression, and tumor growth/oncogenesis. |
| Molecular mechanism: |
circACTN4 upregulates MYC; MYC interacts with TIP60 to increase TIP60 recruitment to MYC target genes and enhance histone H4 acetylation (AcH4), elevating CCNE1/CDK4 expression and promoting cell cycle progression and tumorigenesis. |
| Biological pathway or process: |
cell cycle (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (other); other pathway/process (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; FISH / smFISH; ISH (In Situ Hybridization); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Transfection; CCK8; EdU Staining; Transwell Assay; Cell Cycle Assay; Western Blot; Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
Upregulated circACTN4 expression was significantly related to T stage and TNM stage and poor prognosis (shorter overall survival) in breast cancer patients; potential diagnostic/therapeutic marker. |
| Description: |
circACTN4 is up-regulated in breast cancer and mainly localizes to the nucleus. It promotes proliferation, migration/invasion and xenograft tumor growth by increasing MYC expression, enhancing TIP60-dependent histone H4 acetylation at MYC target genes (e.g., CCNE1 and CDK4), thereby driving G1/S cell cycle progression; high circACTN4 is associated with poorer overall survival. |
| Confidence score: |
0.8595 |