| Expression pattern: |
UP |
| Associated gene: |
MHC-I, H-2-Kd, BMDCs, CD4+ T cells, CD8+ T cells, circGigyf2(82-104), circGigyf2(95-103) |
| Associated microRNA: |
- |
| Biological function: |
Encodes cryptic antigenic peptides that induce antigen-specific T cells and inhibit 4T1 breast cancer tumour growth and lung metastasis when used as peptide or circRNA vaccine; circGigyf2 did not influence proliferation, apoptosis or migration of mouse breast cancer cells. |
| Molecular mechanism: |
Noncanonical translation of circGigyf2 generates cryptic antigenic peptides, including circGigyf2(82-104) and the MHC-I epitope circGigyf2(95-103), which elicit antigen-specific cytotoxic T-cell responses against 4T1 breast cancer. |
| Biological pathway or process: |
immune regulation (promotes); proliferation (inhibits); metastasis (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RT-qPCR; RNA-seq; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; Western Blot; Bioinformatics Analysis; Flow Cytometry（Non-apoptosis/cycle） |
| Clinical significance: |
- |
| Description: |
Mouse circGigyf2 is a cytoplasmic tumour-specific circRNA abundantly expressed in 4T1 breast cancer cells. It encodes cryptic antigenic peptides that generate antigen-specific T-cell responses, and circGigyf2 peptide or RNA vaccination reduces 4T1 tumour growth and lung metastases. |
| Confidence score: |
0.6181 |