| Expression pattern: |
UP |
| Associated gene: |
USF1, TGF-beta1, ESRP1, AGO2 |
| Associated microRNA: |
miR-148a-3p, miR-152-3p |
| Biological function: |
promotes breast cancer cell invasion and metastasis by inducing epithelial-to-mesenchymal transition (EMT); no effect on breast cancer growth |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-148a-3p and miR-152-3p to up-regulate USF1, which transcriptionally up-regulates TGF-beta1 and activates TGF-beta1/Smad signaling to induce EMT; circANKS1B biogenesis is promoted by ESRP1, forming an ESRP1/circANKS1B/miR-148a/152-3p/USF1 feedback loop |
| Biological pathway or process: |
metastasis (promotes); invasion (promotes); EMT (promotes); TGF-beta/SMAD (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); ChIP / ChIP-seq; In Vivo Animal Model; H&E Staining; Clinical Sample Validation; Cohort Study; Survival Analysis |
| Clinical significance: |
High circANKS1B expression is associated with lymph node metastasis and advanced clinical stage, and is an independent predictor of worse overall survival in breast cancer patients |
| Description: |
circANKS1B is up-regulated in TNBC and promotes invasion/metastasis by inducing EMT. It sponges miR-148a-3p and miR-152-3p to de-repress USF1, which up-regulates TGF-beta1 and activates TGF-beta/SMAD signaling; ESRP1 promotes circANKS1B biogenesis, forming a pro-metastatic feedback loop. |
| Confidence score: |
0.8945 |