circRNA basic information
circBase ID: hsa_circ_0058358
Name: hsa_circ_ATG9A
Synonym: circATG9A
Host Gene: ATG9A
Genomic location(hg19): chr2:220088590-220090294:-
Genomic location(hg38): chr2:219223868-219225572:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005086
MONDO name: renal cell carcinoma
Disease details: renal cell carcinoma
Disease DO ID:
 4450
Disease MeSH ID:
 D002292
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

ccRCC tissues; adjacent normal tissues; tumor tissues
Cell lines:

A498; 786-O; HK-2
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: TRPM3, Ago2, c-myc, Cyclin D1, beta-catenin, N-cadherin, Vimentin, MMP2, E-cadherin
Associated microRNA: miR-497-5p
Biological function: circATG9A promotes RCC progression, proliferation, migration, invasion, tumor growth, and epithelial-mesenchymal transition.
Molecular mechanism: circATG9A acts as a miR-497-5p sponge to regulate TRPM3 expression and promotes EMT through the Wnt/beta-catenin signaling pathway.
Biological pathway or process:

Wnt/beta-catenin (promotes); EMT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (other)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; circRNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; Survival Analysis; Bioinformatics Analysis
Clinical significance:

circATG9A expression was significantly correlated with Fuhrman grade, tumor size, TNM stage, and prognosis of RCC patients; it may serve as a potential diagnostic, prognostic, and therapeutic target.
Description:

circATG9A is a human circRNA derived from ATG9A and is up-regulated in RCC/ccRCC tissues and RCC cell lines. It promotes RCC cell proliferation, migration, invasion, tumor growth, and EMT by sponging miR-497-5p to regulate TRPM3 and activate the Wnt/beta-catenin pathway. Clinically, circATG9A expression is associated with tumor features and prognosis, suggesting potential value as a prognostic and therapeutic target.
Confidence score:

0.8933
Other information
Title:

Identification of circATG9A as a novel biomarker for renal cell carcinoma.

Journal: Cancer gene therapy
Published: 2024
PubMed ID: 37945969
Study type:

combined biological and clinical study
Data availability: available from the corresponding author upon reasonable request; supplementary material available at https://doi.org/10.1038/s41417-023-00684-1; Table S2
Code availability: -