| Expression pattern: |
UP |
| Associated gene: |
FUS, ATF3 mRNA, ATF3, SLC7A11 |
| Associated microRNA: |
- |
| Biological function: |
Triggers hippocampal neuronal ferroptosis and promotes epilepsy progression; knockdown of circSLC8A1 suppresses Mg2+-free-induced HN-h cell injury and ferroptosis. |
| Molecular mechanism: |
circSLC8A1 recruits the RNA-binding protein FUS to stabilize ATF3 mRNA expression, thereby promoting neuronal ferroptosis and contributing to epilepsy progression. |
| Biological pathway or process: |
ferroptosis (promotes); mRNA stability (promotes); apoptosis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; IF (Immunofluorescence); RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Western Blot; IHC (Immunohistochemistry); TUNEL; In Vivo Animal Model; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Serum circSLC8A1 was reported as an accurate diagnostic biomarker for epilepsy and a potential therapeutic target. |
| Description: |
circSLC8A1 is up-regulated in epilepsy patient serum, Mg2+-free-induced HN-h epilepsy cell models, and epilepsy rat hippocampal tissues. It promotes hippocampal neuronal ferroptosis by recruiting the RNA-binding protein FUS to stabilize ATF3 mRNA, and serum circSLC8A1 may serve as a diagnostic biomarker and therapeutic target for epilepsy. |
| Confidence score: |
0.8655 |