| Expression pattern: |
UP |
| Associated gene: |
HK1 |
| Associated microRNA: |
miR-520 h |
| Biological function: |
Promotes CRC cell proliferation, growth, angiogenesis, migration, invasion, metastasis, glycolysis and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
circ_0053277 acts as a miR-520 h sponge to positively regulate HK1 expression, thereby promoting CRC progression and glycolysis through the miR-520 h/HK1 axis. |
| Biological pathway or process: |
proliferation (promotes); angiogenesis (promotes); migration (promotes); invasion (promotes); metastasis (promotes); glycolysis (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; Tube Formation Assay; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
circ_0053277 might be a potential clinical target for CRC treatment. |
| Description: |
circ_0053277 is up-regulated in colorectal cancer tissues and cell lines. It functions as an oncogenic circRNA by sponging miR-520 h to increase HK1 expression, thereby promoting CRC cell proliferation, angiogenesis, migration, invasion, metastasis, glycolysis and in vivo tumor growth while suppressing apoptosis. The study suggests circ_0053277 as a potential therapeutic target for CRC. |
| Confidence score: |
0.844 |