| Expression pattern: |
UP |
| Associated gene: |
PI3K, AKT, JAK2, STAT3 |
| Associated microRNA: |
miR-1276 |
| Biological function: |
promotes proliferation, invasion and migration; sustains survival and inhibits apoptosis; promotes tumorigenesis in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-1276, thereby promoting activation of PI3K/AKT and JAK2/STAT3 signaling. |
| Biological pathway or process: |
PI3K/AKT (promotes); JAK/STAT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); Clinical Sample Validation; In Vivo Animal Model; Survival Analysis |
| Clinical significance: |
High circRNA-CIRH1A expression was significantly associated with larger tumor size and more advanced clinical stages, and was associated with a poorer prognosis in OS patients. |
| Description: |
circRNA-CIRH1A (circ_0007018) is up-regulated in osteosarcoma tissues and cell lines and promotes malignant phenotypes (proliferation, migration, invasion) while inhibiting apoptosis, including enhanced tumorigenesis in a U2OS xenograft model. Mechanistically, it acts predominantly in the cytoplasm as a sponge for miR-1276, leading to activation of PI3K/AKT and JAK2/STAT3 signaling. High circRNA-CIRH1A expression is associated with advanced clinicopathological features and poorer overall survival. |
| Confidence score: |
0.8232 |