| Expression pattern: |
DN |
| Associated gene: |
EIF4A3, RGMA, AGO2 |
| Associated microRNA: |
hsa-miR-432-5p |
| Biological function: |
inhibits proliferation, migration, invasion, metastasis and tumor growth; acts as a tumor suppressor gene in LSCC |
| Molecular mechanism: |
EIF4A3 binds to flanking sequences and reduces hsa_circ_0001445 expression; hsa_circ_0001445 acts as a ceRNA sponge for hsa-miR-432-5p to relieve repression of RGMA and up-regulate RGMA expression |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Colony Formation Assay; Transwell Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
low expression is associated with advanced clinical stage, cervical lymph node metastasis and poor overall survival; may be used as a potential prognostic biomarker and therapeutic target for LSCC |
| Description: |
hsa_circ_0001445 is down-regulated in LSCC tissues and cell lines and lower expression is associated with aggressive clinicopathological features and poor overall survival. Functionally, hsa_circ_0001445 suppresses LSCC cell proliferation, migration, invasion and xenograft tumor growth. Mechanistically, EIF4A3 reduces hsa_circ_0001445 expression, while hsa_circ_0001445 acts as a cytoplasmic ceRNA for hsa-miR-432-5p to relieve repression of RGMA. |
| Confidence score: |
0.881 |