| Expression pattern: |
UP |
| Associated gene: |
PTK2, HuR |
| Associated microRNA: |
miR-505-3p |
| Biological function: |
circ_0082319 promotes HCC cell proliferation, invasion, angiogenesis, glycolysis and tumor growth, and suppresses apoptosis; circ_0082319 knockdown represses these malignant phenotypes. |
| Molecular mechanism: |
HuR upregulates circ_0082319 expression; circ_0082319 functions as a miR-505-3p sponge to increase PTK2 expression, thereby accelerating HCC malignant behaviors and glycolysis. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); glycolysis (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Survival Analysis; ROC Analysis; Bioinformatics Analysis; Transfection; MTT; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
circ_0082319 is elevated in HCC tissues and serum; high circ_0082319 expression is associated with lower overall survival, and the combination of circ_0082319 and PIVKA-II has higher diagnostic accuracy for HCC than either marker alone. |
| Description: |
circ_0082319 is up-regulated in HCC tissues, serum and cell lines and is associated with poorer overall survival and potential diagnostic value. Mechanistically, HuR enhances circ_0082319 expression, and circ_0082319 promotes HCC progression by sponging miR-505-3p to elevate PTK2, thereby increasing proliferation, invasion, angiogenesis, glycolysis and tumor growth while inhibiting apoptosis. |
| Confidence score: |
0.8824 |