circRNA basic information
circBase ID: hsa_circ_0000851
Name: hsa_circ_TCF4
Synonym: -
Host Gene: TCF4
Genomic location(hg19): chr18:52926327-52926446:+
Genomic location(hg38): chr18:55259096-55259215:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

TNBC tissues; paired non-tumour tissues; xenograft tumour tissues
Cell lines:

MCF-10A; MDA-MB-231; BT-549; MDA-MB-468; MCF-7; 293 T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PDK1, p-AKT, AGO2
Associated microRNA: miR-1183
Biological function: Promotes TNBC cell proliferation, migration, and xenograft tumour growth; knockdown reduces proliferation and migration.
Molecular mechanism: hsa_circ_0000851 acts as a competing endogenous RNA by sponging miR-1183, thereby increasing PDK1 expression and activating AKT phosphorylation.
Biological pathway or process:

PI3K/AKT (promotes); proliferation (promotes); migration (promotes); ceRNA regulation (other)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

hsa_circ_0000851 expression correlated positively with Ki-67, tumour size, and lymph node metastasis; it may be a potential biomarker and therapeutic target for TNBC.
Description:

hsa_circ_0000851 is a TCF4-derived circRNA upregulated in breast cancer, particularly TNBC tissues and cell lines. It functions as an oncogenic cytoplasmic ceRNA that sponges miR-1183, increases PDK1 expression, activates p-AKT signaling, and thereby promotes TNBC proliferation, migration, and xenograft tumor growth. Its expression is associated with Ki-67, tumor size, and lymph node metastasis, suggesting potential biomarker and therapeutic-target value.
Confidence score:

0.8537
Other information
Title:

Hsa_circ_0000851 promotes PDK1/p-AKT-mediated cell proliferation and migration by regulating miR-1183 in triple-negative breast cancer.

Journal: Cellular signalling
Published: 2023
PubMed ID: 36241055
Study type:

combined biological and clinical study
Data availability: circBase (http://circrna.org/); UCSC Genome Browser (http://genome.ucsc.edu/); Circular RNA Interactome (https://circinteractome.nia.nih.gov/index.html); starBase v2.0 (https://starbase.sysu.edu.cn/); miRanda (http://www.microrna.org/); RNAhybird (http://bibiserv.techfak.uni-bielefeld.de/rnahybrid/); circAtlas (http://circatlas.biols.ac.cn/); Supplementary data: https://doi.org/10.1016/j.cellsig.2022.110494
Code availability: -