| Expression pattern: |
UP |
| Associated gene: |
METTL3 |
| Associated microRNA: |
miR-590-5p |
| Biological function: |
promotes NSCLC cell growth, proliferation, cell cycle progression, glycolysis, and xenograft tumor growth |
| Molecular mechanism: |
ceRNA mechanism: circPUM1 sequesters miR-590-5p and up-regulates METTL3 expression |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); glycolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Western Blot; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circPUM1 expression was associated with advanced TNM stages and shorter overall survival in patients with NSCLC |
| Description: |
circPUM1 is up-regulated in NSCLC tissues and cell lines, and high expression is associated with advanced TNM stage and poorer overall survival. Functionally, circPUM1 promotes NSCLC proliferation, cell cycle progression, glycolysis and xenograft tumor growth by sponging miR-590-5p and increasing METTL3 expression. |
| Confidence score: |
0.8322 |