circRNA basic information
circBase ID: -
Name: hsa_circ_AGFG1
Synonym: circular RNA AGFG1
Host Gene: AGFG1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BC tissues; normal tissues; tumor tissues
Cell lines:

MCF-10A; MDA-MB-231; MCF-7; SK-BR-3; MDA-MB-157
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: YWHAE, 14-3-3 protein epsilon, MMP9, GLUT1, Bax, Ki67
Associated microRNA: miR-653-5p
Biological function: Promotes breast cancer cell proliferation, invasion, migration, glycolysis, and tumor growth; inhibits apoptosis.
Molecular mechanism: circAGFG1 acts as a ceRNA sponge for miR-653-5p to mediate YWHAE expression, affecting MMP9, GLUT1 and Bax expression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glycolysis (promotes); metastasis (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Cohort Study
Clinical significance:

High circAGFG1 expression was associated with tumor-node-metastasis stage, tumor size, lymph node metastasis, distant metastasis, and worse overall survival in BC patients.
Description:

circAGFG1 is up-regulated in breast cancer tissues and cell lines and its high expression is associated with aggressive clinicopathological features and poor overall survival. Functionally, circAGFG1 promotes proliferation, migration, invasion, glycolysis, and in vivo tumor growth while suppressing apoptosis. Mechanistically, it acts as a sponge for miR-653-5p to regulate YWHAE and downstream proteins including MMP9, GLUT1, and Bax.
Confidence score:

0.8658
Other information
Title:

Circular RNA AGFG1 motivates breast cancer cell proliferation, invasion, migration, and glycolysis by controlling microRNA-653-5p/14-3-3 protein epsilon.

Journal: Acta biochimica Polonica
Published: 2023
PubMed ID: 37851953
Study type:

combined biological and clinical study
Data availability: -
Code availability: -