| Expression pattern: |
UP |
| Associated gene: |
MCL1, SNAI2, AGO2 |
| Associated microRNA: |
miR-320b |
| Biological function: |
promotes cell proliferation; inhibits apoptosis |
| Molecular mechanism: |
circMTO1 sponges miR-320b to regulate MCL1 expression; circMTO1 expression is transcriptionally induced by SNAI2 via MTO1 promoter binding |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; ChIP / ChIP-seq |
| Clinical significance: |
- |
| Description: |
In insulin-treated human granulosa-like tumor cells (PCOS cellular model), circMTO1 is upregulated and mainly cytoplasmic. circMTO1 promotes proliferation and inhibits apoptosis by acting as a ceRNA that sponges miR-320b to upregulate MCL1, and its expression is induced by the transcription factor SNAI2 via the MTO1 promoter. |
| Confidence score: |
0.7836 |