circRNA basic information
circBase ID: hsa_circ_0007766
Name: hsa_circ_ERBB2
Synonym: -
Host Gene: ERBB2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0003219
MONDO name: gastroesophageal junction adenocarcinoma
Disease details: adenocarcinoma of the esophagogastric junction
Disease DO ID:
 4944
Disease MeSH ID:
 -
Disease NCIt ID:
 C9296
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

AEG tissues; matching healthy gastric mucosal tissues; adjacent normal mucosal tissues; noncancerous gastric mucosal tissues; paraffin-embedded AEG tissues; frozen tissues
Cell lines:

HGC-27; AGS; SK-GT-4; GES-1; 293 T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: cyclin D1, ERBB2, ki-67, N-cadherin, beta-catenin, E-cadherin, c-Myc
Associated microRNA: miR-34c-5p
Biological function: Promotes AEG progression, tumorigenesis, proliferation, migration, invasion, cell cycle progression and EMT-related protein changes, while inhibiting apoptosis.
Molecular mechanism: circRNA0007766 functions as a ceRNA that sponges miR-34c-5p, thereby upregulating cyclin D1 at the post-transcriptional/protein level and promoting AEG malignant progression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); EMT (promotes); metastasis (promotes)
Detected method:
Q
H
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; FISH / smFISH; ISH (In Situ Hybridization); Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Survival Analysis; ROC Analysis; Bioinformatics Analysis
Clinical significance:

circRNA0007766 upregulation is correlated with lymph node involvement, poor prognosis, short overall survival, and may serve as a specific and sensitive diagnostic biomarker and potential novel AEG biomarker.
Description:

circRNA0007766 is a human ERBB2-derived circRNA that is significantly upregulated in AEG tissues and cell lines. It promotes AEG proliferation, migration, invasion, tumorigenesis, cell cycle progression and EMT-related changes while reducing apoptosis, mainly through a ceRNA mechanism in which it sponges miR-34c-5p to upregulate cyclin D1. Clinically, high circRNA0007766 expression is associated with lymph node metastasis, poor prognosis and shorter overall survival, suggesting diagnostic and prognostic biomarker potential.
Confidence score:

0.8648
Other information
Title:

CircRNA0007766 accelerates cancer progression via miR-34c-5p/cyclin D1 axis in adenocarcinoma of the esophagogastric junction (AEG).

Journal: Cellular signalling
Published: 2023
PubMed ID: 37802173
Study type:

combined biological and clinical study
Data availability: Data will be made available on request; Supplementary data to this article can be found online at https://doi. org/10.1016/j.cellsig.2023.110912.
Code availability: -