circRNA basic information
circBase ID: hsa_circ_0001258
Name: -
Synonym: -
Host Gene: -
Genomic location(hg19): chr22:50810448-50832564:+
Genomic location(hg38): chr22:50372019-50394135:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

OS tissues; primary OS specimens
Cell lines:

MG63; KHOS; U2OS; MG63/DXR; KHOS/DXR; U2OS/DXR; KH-OS/DXR
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: GSTM2
Associated microRNA: hsa-miR-744-3p
Biological function: hsa_circ_0001258 suppressed doxorubicin resistance of osteosarcoma cells and regulated cell viability and IC50 value to doxorubicin.
Molecular mechanism: ceRNA mechanism: hsa_circ_0001258 sponges hsa-miR-744-3p to upregulate GSTM2 expression.
Biological pathway or process:

ceRNA regulation (other); drug resistance (inhibits); chemoresistance (inhibits)
Detected method:
Q
S
Validation methods:

RT-qPCR; RNA-seq; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Luciferase Reporter Assay; Western Blot
Clinical significance:

Potential novel target for reversing osteosarcoma chemo-resistance; expression validated in chemo-resistant and chemo-sensitive OS tissues.
Description:

hsa_circ_0001258 is down-regulated in chemo-resistant osteosarcoma cell lines and tissues. It suppresses doxorubicin resistance through a ceRNA mechanism by sponging hsa-miR-744-3p and increasing GSTM2 expression.
Confidence score:

0.6676
Other information
Title:

Analyzing the Interactions of mRNAs and ncRNAs to Predict Competing Endogenous RNA Networks in Osteosarcoma Chemo-Resistance.

Journal: Molecular therapy : the journal of the American Society of Gene Therapy
Published: 2019
PubMed ID: 30692017
Study type:

combined biological and clinical study
Data availability: Supplemental Information includes nine tables; https://doi.org/10.1016/j.ymthe.2019.01.001
Code availability: -