| Expression pattern: |
DN |
| Associated gene: |
PPM1L |
| Associated microRNA: |
miR-3180-5p, miR-196a-3p, miR-203a-3p |
| Biological function: |
circ_0061235 is a serum diagnostic biomarker candidate for NSCLC; its decreased levels are associated with lymph node metastasis, distant metastasis, and advanced tumor stages, and its combination with miR-3180-5p and PPM1L improves diagnostic sensitivity and specificity. |
| Molecular mechanism: |
Predicted ceRNA mechanism in which circ_0061235 may sequester miR-3180-5p, thereby affecting PPM1L expression; bioinformatics analyses linked the axis to tumor progression pathways including PI3K-Akt signaling, Ras signaling, and regulation of actin cytoskeleton. |
| Biological pathway or process: |
ceRNA regulation (other); PI3K/AKT (other); metastasis (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ_0061235 is significantly downregulated in NSCLC serum and has diagnostic value for distinguishing healthy individuals or benign lung tumors from NSCLC; reduced circ_0061235 is associated with lymph node metastasis, distant metastasis, and higher tumor stages. |
| Description: |
This study identified serum circ_0061235 as a downregulated circRNA in NSCLC and established a circ_0061235-miR-3180-5p-PPM1L ceRNA network. The axis has diagnostic value for distinguishing NSCLC from healthy controls and benign lung tumors, and low circ_0061235 levels are associated with more aggressive NSCLC features including lymph node metastasis, distant metastasis, and advanced stage. |
| Confidence score: |
0.459 |